Plasmid

Part:BBa_K4823012:Design

Designed by: Zike Yao   Group: iGEM23_HKSSC   (2023-10-10)


PA1897_EGFP_mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 789
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1724


Design Notes

we have characterized it to (correcting) PA1897 as promoter. We have knocked out the Lac operon to ensure the EGFP gene is fully regulated.


Source

Escherichia coli BL21

References